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KMID : 1149220180260020103
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Korean Journal of Oriental Medical Prescription
2018 Volume.26 No. 2 p.103 ~ p.111
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Antioxidant Effect of Rubi Fructus on TM4 Sertoli Cells
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Kim Young-Joo
Chang Mun-Seog
Park Seong-Kyu
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Abstract
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Objectives : The purpose of this study was to examine the antioxidant effects of the extract of Rubi Fructus on TM4 Sertoli cells.
Methods : The extract was studied for diphenyl-picryl-hydrazyl (DPPH) radical scavenging activity and cell viability assays on Sertoli cells. In addition, hydrogen peroxide-induced oxidative stress on Sertoli cells were examined by MTT assay. The antioxidant enzyme of Cu/Zn SOD, Mn SOD, catalase protein expression on Sertoli cells were also measured.
Results : The results showed that the extract scavenged DPPH radical dose-dependent manner. The extract showed no cytotoxicity at concentration of 1, 5, 10, 50, 100 μg/ml. The hydrogen peroxide-induced cytotoxicity of Sertoli cells was protected to 88.3% by the extract at concentration of 100 μg/ml. Cu/Zn SOD and Mn SOD protein expression were significantly increased on Sertoli cells, but catalase protein expression was not significantly changed.
Conclusions : In conclusion, the extract of Rubi Fructus has antioxidant effects on Sertoli cells and protect male reproductive system against oxidative stress.
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KEYWORD
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Rubi Fructus , Sertoli cells , diphenyl-picryl-hydrazyl (DPPH) , MTT assay , hydrogen peroxide , Cu/Zn SOD , Mn SOD , catalase
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